Protocols

I, DNA/RNA Isolation
        1. DNA/RNA Isolation from Tissues
            a, RNA isolation from human tissues
            b, RNA isolation from human coronary artery speciments
            c, RNA isolation from mouse tissues

        2. DNA/RNA Isolation from Cultured Cells

II. Microarray
        1. Sample Preparation
            a, mouse array
            b, human array
        2. Hybridization
            a, mouse array
            b, human array
        3. Analysis

III. Tissue Culture
        1. Endothelial Stem Cells
        2. Endothelial Cells
        3. Smooth Muscle Cells
        4. Transfected COS Cells
        5. Stimulation

IV. Vectors and Constructs
        1. Vectors
            a. SuperTie2 and map
        2. Constructs

V. Histology
        1. DiI_Ac-LDL

VI. In Situ

Protocols:

- DNA/RNA
  Isolation

- Microarray

- Tissue Culture

- Vector and
  Constructs

- Histology

- In Situ